Journal: Nature communications

Article Title: A specialized bone marrow microenvironment for fetal haematopoiesis.

doi: 10.1038/s41467-022-28775-x

Figure Lengend Snippet: Fig. 7 Wnt2 promotes HSPC proliferation and transplantation. a Representative FACS plot showing transient EdU incorporation into sorted LSK cells after treatment with Wnt2 or vehicle. Quantification of EdU% in LSK cells. Ctrl = 6; Wnt2 = 7. Error bars, mean ± s.e.m. Two-tailed Student’s t test. b Representative images (8 days after seeding) and quantification of colony forming units from 600 isolated wild-type adult LSK cells. For colony count, vehicle = 10; Wnt2 = 9. For total cell number count, vehicle = 6; Wnt2 = 5. Error bars, mean ± s.e.m. Two-tailed Student’s t test. c, d Quantification of HSC number and LSK number fold change relative to adult cell input (c); vehicle = 10; Wnt2 = 9. Absolute cell number after Wnt2 treatment (d); vehicle = 15; Wnt2 = 14. Error bars, mean ± s.e.m. Two-tailed Student’s t test. e Diagram showing Wnt2-treatment of sorted adult Vav1-mTmG LSK cells in vitro followed by analysis at 5 days after transplantation into lethally-irradiated WT mice. f Representative overview and high-magnification immunostaining results showing hosts bone sections at 5 days after transplantation with Wnt2 or vehicle-pretreated GFP+ cells or fresh input cells. g FACS plot showing GFP+ or GFP+ CD11b+ cells from hosts at 5 days after transplantation with Wnt2 or vehicle-pretreated GFP+ cells or fresh input cells. h FACS based quantification of GFP+, GFP+ CD45+ and GFP+ CD11b+ cell percentage in BM at 5 days after transplantation. Input = 5; ctrl = 20; Wnt2 = 15. Error bars, mean ± s.e.m. Two-tailed Student’s t test. i Diagram showing Wnt2-treatment of sorted adult WT LSK cells in vitro followed by analysis at 2 weeks after transplantation into lethally-irradiated WT mice. j FACS based quantification of LSK%, SLAM-LSK% (ctrl = 10; Wnt2 = 10) as well as the number of BMNCs, Gr-1+

Article Snippet: The remaining cells were then incubated with CD117 (Miltenyi Biotec, 130-091-224, 10 μl per 107 cells), CD45 (Miltenyi Biotec, 130-052-301, 10 μl per 107 cells) and Ter119 (Miltenyi Biotec, 130-049-901, 10 μl for 107 cells) antibodies for a final lineage separation to maximize the cells of interest.

Techniques: Transplantation Assay, Two Tailed Test, Isolation, In Vitro, Irradiation, Immunostaining

Journal: Cell

Article Title: Intrinsic Immunity Shapes Viral Resistance of Stem Cells

doi: 10.1016/j.cell.2017.11.018

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The other fraction was further diluted in appropriate volume (80 μl/10 million cells) of CD45 selection buffer (PBS, 0.5% BSA and 2mM EDTA) with CD45 microbeads (Miltenyi Biotec,20 μl/10 million cells) and incubated at 4°C for 15 min . CD45 − cells were isolated by immunomagnetic negative selection according to the manufacturer’s instructions.

Techniques: Control, Recombinant, Clinical Proteomics, Knock-Out, Membrane, SYBR Green Assay, Sample Prep, Selection, shRNA, Software, Gene Expression

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

Article Title: Early stage drug treatment that normalizes proinflammatory cytokine production attenuates synaptic dysfunction in a mouse model that exhibits age-dependent progression of Alzheimer's disease-related pathology

doi: 10.1523/JNEUROSCI.1496-12.2012

Figure Lengend Snippet: Selective effects of MW-151 on inflammation-related gene expression markers

Article Snippet: Relative gene expression was calculated by the 2 −ΔΔCT method. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Δ >20% p<0.05 veh MW-151 gene/taqman assay ID IBA1 ⬇ 100±9 75±4 Aif1/Mm00479862_g1 CD45 100±8 88±10 Ptprc/Mm01293575_m1 CD68 ⬇ 100±9 74±2 Cd68/Mm00839636_g1 TLR2 100±13 118±17 Tlr2/Mm00442346_m1 TLR4 ⬆ 100±15 150±34 Tlr4/Mm00445273_m1 NOS2 100±15 113±18 Nos2/Mm00440502_m1 TNFα ⬇ 100±12 62±18 Tnf/Mm00443258_m1 TGFβ ⬇ 100±7 75±3 Tgfb1/Mm00441726_m1 IGF1 ⬇ 100±28 76±17 Igf1/Mm00439561_m1 CD200 ⬆ 100±9 218±57 Cd200/Mm00487740_m1 fractalkine ⬆ 100±9 134±6 Cx3cl1/Mm00436454_m1 HMGB1 ⬇ 100±9 70±3 Hmgb1/Mm00849805_gH Open in a separate window Selective effects of MW-151 on inflammation-related gene expression markers

Techniques: Gene Expression